In recent years, several massive toxicological analyses have yielded large genomic data sets obtained with DNA-microarrays, Next generation sequencing and PCR studies. Real-time PCR is becoming a common tool for detecting and quantifying expression profiling of selected genes. Anthelos developed molecular diagnostic solutions to predict drug efficacy and side effects based on gene expression profiling with multiplex QPCR technology.
Our focused QPCR panels possess high specificity and sensitivity. The reproducibility of both the Toxicogenomic and the Cytokine panels is high: the reagent kits demonstrate strong correlations across technical replicates, QPCR instruments and production lots with average correlation coefficients >0.99, ensuring reliable detection of differences in marker gene expression between biological samples.
In addition to genotoxicity and mutagenicity testing, one promising approach consists in the evaluation of mRNA expression profiles provoked by the respective drugs or drug candidates. The genes in the Toxicology gene panel were selected to be the best predictors for toxic side effects and cellular stress, including among others are ATF4, HSP90AA1, HMOX1, GADD45, NQO1 and DDIT3.
Cytokine gene expression quantification is widely used in immunological research to dissect the early steps of immune responses or pathophysiological pathways. The genes in the Cytokine gene panel were selected to be the best predictors for inflammatory signals and stimulation, including among others are IL1B, INFG, IL6, TNF, INFA1, IL2 and IL8.
Ant-06 Toxicology gene panel – rat (100)
Ant-07 Cytokine gene panel – rat (100)
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